PURPOSE
To measure the cohesive tensile strength of human LASIK corneal wounds.
METHODS
Twenty-five human eye bank corneas from 13 donors that had LASIK were cut into 4-mm corneoscleral strips and dissected to expose the interface wound. Using a motorized pulling device, the force required to separate the wound was recorded. Intact and separated specimens were processed for light and electron microscopy. Five normal human eye bank corneas from 5 donors served as controls. A retrospective clinical study was done on 144 eyes that had LASIK flap-lift retreatments, providing clinical correlation.
RESULTS
The mean tensile strength of the central and paracentral LASIK wounds showed minimal change in strength over time after surgery, averaging 2.4% (0.72 ± 0.33 g/mm) of controls (30.06 ± 2.93 g/mm). In contrast, the mean peak tensile strength of the flap wound margin gradually increased over time after surgery, reaching maximum values by 3.5 years when the average was 28.1% (8.46 ± 4.56 g/mm) of controls. Histologic and ultrastructural correlative studies found that the plane of separation always occurred in the lamellar wound, which consisted of a hypocellular primitive stromal scar centrally and paracentrally and a hypercellular fibrotic stromal scar at the flap wound margin. The pathologic correlations demonstrated that the strongest wound margin scars had no epithelial cell ingrowth—the strongest typically being wider or more peripherally located. In contrast, the weakest wound margin scars had epithelial cell ingrowth. The clinical series demonstrated the ability to lift LASIK flaps without complications during retreatments up to 8.4 years after initial surgery, correlating well with the laboratory results.
CONCLUSIONS
The human corneal stroma typically heals after LASIK in a limited and incomplete fashion; this results in a weak, central and paracentral hypocellular primitive stromal scar that averages 2.4% as strong as normal corneal stroma. Conversely, the LASIK flap wound margin heals by producing a 10-fold stronger, peripheral hypercellular fibrotic stromal scar that averages 28.1% as strong as normal corneal stromal, but displays marked variability. [J Refract Surg. 2005;21:433-445.]
AUTHORS
From the Emory Eye Center, Emory University School of Medicine, Atlanta, Ga (Schmack, Dawson, McCarey, Grossniklaus, Edelhauser); the Department of Ophthalmology, Ruprecht-Karls-University, Heidelberg, Germany (Schmack); and InView, Atlanta, Ga (Waring).
Drs Schmack and Dawson contributed equally to this work.
Supported in part by NEI grants: EY-00933, P30-EY06360, T32-EY07092, and an unrestricted departmental grant from Research to Prevent Blindness.
The authors have no proprietary interest in the materials presented herein.
The authors thank Patrick DeLeon for the photoediting, Nancy L’Hernault for the electron microscopic studies, and Gregg Russell, OD, from InView Refractive Surgery Center for help compiling the clinical data.
Correspondence: Henry F. Edelhauser, PhD, Emory Eye Center, 1365-B Clifton Rd NE, Atlanta, GA 30322. Tel: 404.778.5854; Fax: 404.778.4143; E-mail: ophthfe@emory.edu
Received: April 25, 2005
Accepted: May 24, 2005
